Invitrogen TOPO TA Cloning Kit, with pCR2. 1TOPO, One Shot TOP10 Chemically Competent E. coli, and PureLink Quick Plasmid Miniprep Kit 25 reactions Practicals 5& 6 Molecular Cloning of PCR Products (Instructors: Drs.
Sze Chun Chau& Valerie C. L. Lin) Cloning of PCR products into TOPO TA vectors pCR2. 1 TOPO contains a 115 bp polylinker with cloning sites for 16 restriction enzymes PCR2. 1 Topo Reaction See the Topo TA cloning A1360 and the Invitrogen TOPO TA Cloning Kit with pCR2.
1TOPO. The TOPO manual indicates that any PCR product An expression cassette was assembled onto the pCR2. 1Topo vector Genetic transformation of Find and Purchase TOPO TA Cloning Kit with PCR2. 1 TOPO Products at Invitrogen Life Science Technologies. PCR2. 1 Topo Reaction See the Topo TA cloning manual for information.
The vector PCR2. 1 topo is 3, 900bp and supplied at 10ngul. You will use 1ul of PCR2. 1 topo which is 0. pmoles. With your calculated insert from step 3, assemble the topo reaction. The topo ran is 6ul 1ul PCR2. 1 topo 1ul salt solution Xul insert from step 4. Y Topo Ta Cloning Protocol Invitrogen the TOPO TA cloning Kit with our pCR2. 1 TOPO TA vector are: Environmentally friendly: Save a tree and use your wellloved TOPO Pcr2 1 topo cloning manual treadmill Kit manual.
This kit. Clone this PCR fragment into the vector using the Invitrogen TOPO TA Cloning (Version R) Basic Cloning Vectors CRISPR Plasmids Gateway Cloning Vectors TA and GC Cloning Vectors Fluorescent Protein Genes& Plasmids Luciferase Vectors Mammalian Expression Vectors Viral Expression& Packaging Vectors I. M. A. G. E. Consortium Plasmids pCR2. 1TOPO Yeast Plasmids Plant Vectors Insect Cell Vectors Lucigen Vectors pET& Duet Vectors Vector database is a digital collection of vector backbones assembled from publications and commercially available sources.
This is a free resource for the TOPO TA Cloning Kit (with pCR2. 1TOPO vector supplied with Cat. nos. K and K refer to the manual supplied with the miniprep The TA Cloning Kit with pCR2.
1 vector provides a quick, onestep cloning strategy for directly inserting a Taqamplified PCR product into a plasmid vector. The TA Cloning Kit uses the pCR2. 1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteenminute, roomtempera Invitrogen TOPO TA Cloning Kit for Subcloning, without competent cells 25 reactions directional subcloning. pCR 2. 1TOPO TA clone selection and manipulation The pCR 2. 1TOPO TA vector contains both ampicillin and kanamycin resistance markers and the LacZ Box 1: Topoisomerase Iactivated pCR2.
1TOPO vector, PCR buffer, Salt solution Can I use my insert in pCR 2. 1 TOPO TA Cloning vector for in vitro transcription process by the method of PCR purification? The pCR2. 1TOPO TA vector map shows a T7 promoter sequence but it is